BIO RAD ANN ARBOR MANUAL

This provides multiple copies of each starting template molecule, increasing the chance of each individual molecule getting enriched. To have sufficient unique molecules for good sequencing coverage of your targets, successful captures DEPEND on the input of sufficiently complex libraries.įor best results, it is recommended that only amplified (non-PCR-free) NGS libraries are used for target capture.

Target capture necessarily requires subjecting your libraries to a bottleneck, wherein target molecules are captured and therefore enriched, and non-target molecules are therefore removed. Specific recommendations for per-library input mass for different enrichment project types can be found in the applicable myBaits manual. If you are looking to outsource your project to a full-service laboratory and bioinformatics services group, please visit our myReads NGS laboratory and bioinformatics services page for more information about our comprehensive targeted sequencing service options (library preparation, target capture, next-generation sequencing, and optional analysis).

BIO RAD ANN ARBOR MANUAL

Please review the list of required materials in the applicable myBaits manual to make sure you have everything you need before starting your experiments. Please note that there are some additional reagents and equipment you will need to supply in order to perform a myBaits capture. You will receive enough probes and reagents for performing the stated number of individual capture reactions of your kit size (e.g., 16 reactions) according to our current protocol. For myBaits Custom kits, optional custom probe design informatics service (= expert bioinformaticians design and filter bait sequences and provide summary report and recommendations).Biotinylated RNA probes, with sequences corresponding to your custom design or a predesigned catalog option.

Bio rad ann arbor